Figure 6

Oridonin inhibits colony formation of SW1116 in vitro and tumor growth in vivo. SW1116 cells were treated with 0, 6.25, 12.5, 25, 50, 100 μM oridonin for 14 days. (A) The CFE was defined as the percentage of plated cells that formed colonies relative to an untreated control (untreated cells = 100%). The means ± standard deviations of six independent experiments per oridonin concentration are shown. The symbol * represents P < 0.05 compared with control group. SW1116 cells (1 × 10⁷ cells in 100 μl of PBS) were subcutaneously injected into the right flank of BALB/c nude mice. The mice were then administered intraperitoneally with 0.2 ml of PBS or DMSO (1% in PBS) or Oridonin (6.25, 12.5 and 25 mg/kg respectively) daily when tumors reached a volume of 50-100 mm³. (B) Tumor dimension was periodically measured using calipers over a 4-week period. Each point represents average volume calculated from ten mice. (C) One day after the last treatment, tumors were excised from the animals, and tumor weight was measured. The data are representative of three independent experiments. Symbol * represents P < 0.05 compared with control group. (D) Changes in body weight of mice treated with PBS or DMSO (1% in PBS) or oridonin.