Figure 3

Res repressed YKL-40 promoter activity, mRNA transcription, and protein expression. A. Luciferase assay after YKL-40 promoter reporter plasmid-transfected U87 cells after treatment with 100 μM Res for 48 h. B. YKL-40 mRNA transcription level measured using real-time RT-PCR after treatment with 100 μM Res for 24, 48, and 72 h. C. YKL-40 protein expression levels measured using western blotting after treatment with 100 μM Res for 24, 48, and 72 h. D. Secreted YKL-40 protein levels in media measured using ELISA after treatment with 100 μM Res for 24, 48, or 72 h. Asterisks indicate statistically significant changes (P < 0.01). Bars indicate SE.