Figure 2

Sorafenib induces apoptosis via cleavage of caspases. (A) Apoptotic (Annexin V FITC⁺ and Propidium iodide^-) and necrotic cells (Annexin V FITC⁺ and Propidium iodide⁺) were detected by flow cytometry analysis at 24 h and 48 h following Sorafenib treatment. Results are displayed for SEM cells treated with DMSO or 7.3 μM Sorafenib. (B) Cells were treated as described in Figure 1. Total cell lysates (25 μg) were analyzed by Western blot to detect the cleavage of Caspase 3, 7 and PARP. GAPDH was used as control for equal loading.