Figure 1From: p27Kip1deficiency promotes prostate carcinogenesis but does not affect the efficacy of retinoids in suppressing the neoplastic process Morphological changes in DLP of p27 deficient mice in the course of carcinogenesis. 1A: Dorso-lateral prostate of 6-month-old p27-/- mice. Note the glandular structures are covered by a single layer of prostate epithelial cells (PEC). Glands are surrounded by a thick layer of myoepithelial cells, stroma cells and collagen (arrows). H&E staining × 200. 1B: High grade PIN in a p27-/- mice treated with MNU and sacrificed at the age of 9 months. Hyperplastic and dysplastic PEC cover entire glands and form papillary and alveolar structures (arrows). The surrounding connective tissue stroma is very abundant (arrow had). H&E × 200. 1C: Ductal carcinoma of papillary type in DLP of a p27-/- animal treated with MNU and sacrificed at the age of 10 months. Note, tumor cells occupy the entire glands lumen. H&E × 200. 1D: Infiltrative prostate carcinoma in a p27-/- animal sacrificed at the age of 12 months. Tumor cells infiltrate the surrounding stroma (arrows). H&E × 200. 1E: Pituitary tumor in a p27-/- mice sacrificed at the age of 10 months. H&E × 200. 1F: Intestinal carcinoma in a p27-/- mice sacrificed at the age of 9 months. Note tumor cell infiltration in the surrounding stroma (arrow). H&E × 200. 1G: RARα expression in DLP of a 6-month-old p27+/- mice. RARα was detected by anti-RARα antibody (SC-251) and ABC kit. The receptor is expressed in papillary structures (arrow) and in the basal layer of epithelial cells. The slide is counterstained by hematoxylin × 200. 1H: RXRα expression in PIN of p27-/- 9-month-old mice. Note that most epithelial cells express RXRα receptor (arrow). The slide is counterstained by hematoxylin × 200.Back to article page