Figure 2

Effects of GSK-3 inhibitors on MM cell proliferation. (A, B) Graphs showing representative in vitro kinase assays performed using human recombinant GSK-3β (hrGSK-3β) and total protein lysates (+ = 1 μg, ++ = 4 μg) of OPM-2 cells (A) and RPMI-8226 cells (B) that were cultured with the two specific GSK-3 inhibitors, SB216763 at 10 μM and SB415286 at 4 μM. The graphs show a remarkable inhibition of both hrGSK-3β protein (leftmost graphs) and endogenous GSK-3 kinase activity (rightmost graphs) (y axis: counts per minute). Data represent mean ± SD, n = 3. * indicates p < 0.05 by one-way ANOVA test. (C) Dose-response graphs of U-266 (left) and INA-6 cells (right) incubated for 48 hours with increasing concentrations of SB415286. Cell proliferation was assessed by ³H-thymidine-incorporation assay. Data represent mean ± SD, n = 4, * indicates p < 0.05 by one-way ANOVA test.