Figure 3
From: Molecular targets for the protodynamic action of cis-urocanic acid in human bladder carcinoma cells
Cell cycle progression of 5637 bladder carcinoma cells is inhibited in response to cis-UCA. The cells were synchronized, treated with 0-3% cis-UCA or PIPES (corresponding to 3% cis-UCA equimolar concentration) for 2 h, and let to recover in the absence of treatments for 24 h. After incubation the collected cells were disrupted in sodium citrate buffer and the PI labelled nuclei were analysed by flow cytometry for DNA content. Cell cycle phases were gated and the percentage of cells in each phase quantified. Statistical analysis of the percentage of nuclei in G2/M cell cycle phase is presented (Mean ± SEM; n = 6; Student's t-test n.s. non-significant, ** p < 0.01, *** p < 0.001).