Figure 4

Effect of TGZ on the levels of Akt and p-Akt. (A) Subconfluent LM8 cells were treated for 3 days with TGZ at the indicated concentrations. The proteins (15 μg of protein/lane) in the supernatant of cell homogenates were separated by SDS-PAGE and the expression of Akt and p-Akt was analyzed by Western blot. (B) Subconfluent LM8 cells were treated for 3 days with or without 50 μM TGZ. The expression of Akt and p-Akt was analyzed by Western blot, and relative densitometric units were determined using the analysis software. Values given are the means ± SE for five plates. *p < 0.01 (compared with the untreated cultures).