Figure 2

Effect of TGZ on cell proliferation. (A) Subconfluent LM8 cells were treated for 3 days with 0-50 μM TGZ, and the DNA content of the cultures was measured. Values given are the means ± SE for four plates. *p < 0.01 (compared with the untreated cultures). (B) Subconfluent LM8 cells were treated with (filled circle) or without (open circle) 50 μM TGZ, and the DNA content of the cultures at the indicated intervals was measured. Values given are the means ± SE for four plates. *p < 0.01 (compared with the untreated cultures on the corresponding day). (C) Subconfluent LM8 cells were treated for 3 days with or without 50 μM TGZ in the absence or presence of 10 μM GW9662 and were incubated with 30 μM BrdU during the last 2 hr of the 3-day treatment period. The fixed cells were incubated for 1 h with a mouse monoclonal anti-BrdU antibody followed by a 1 h incubation with an FITC-labeled anti-mouse IgG. The values given in the photographs represent the BrdU-labeling index and are the means ± SE for five determinations. *p < 0.05, **p < 0.01 (compared with the untreated cultures). Magnification: ×100. (D) Subconfluent LM8 cells were treated for 3 days with the indicated additive, and the DNA content of the cultures was measured. Values given are the means ± SE for four plates. *p < 0.01 (compared with the untreated cultures). **p < 0.01 (compared with the cultures treated with TGZ alone).