Figure 5

Prohibition of CHOP induction attenuates RES-induced apoptosis in Raji and Daudi cells. Raji and Daudi cells were treated with the indicated concentration of RES for 24 h and cell viability (A) and apoptotic cells (B) were analyzed using MTT and FACS, respectively. C, Cells were pretreated with vehicle or BAPTA for 3 h, then treated with RES for 24 h and cytosolic calcium was analyzed using flow cytometry. D, Cells were treated as C, and apoptotic cells were analyzed using Annexin V/PI staining followed by flow cytometry. E, Cells were transfected with scramble, mutant siRNA against CHOP (mutsiCHOP) or siRNA against CHOP (siCHOP) on three consecutive days, treated with 100 μM of RES for 24 h and cell lysates were subjected to Western blotting analysis. F, Cells were treated as C and apoptotic cells were analyzed using FACS. G, HMyc2.CIR cells were treated with indicated concentration of RES for 8 h and real-time PCR was performed. H, HMyc2.CIR cells were treated with indicated concentration of RES for 24 h and Western blot analysis was performed. I, HMyc2.CIR cells were treated with 100 μM of RES for 24 h, apoptosis of cells was analyzed using flow cytometry. *, P < 0.01.