Figure 3

Leptin enhances the binding of STAT3, Myc, H3 to hTERT promoter. (a) ChIP assay was performed using anti-STAT3 antibody or irrelevant anti-immunoglobin G antibody as negative control. Input samples are total genomic DNAs used as control for the PCR. PCR primers covering the STAT3 binding sites of hTERT promoter region were used to detect promoter fragment in immunoprecipitates. Leptin (-), untreated cells, Leptin (+), 200 ng/ml for 48 h, Leptin (++), 100 ng/ml for 2 months. (b) ChiP assay was performed using anti-Mad1, anti-Max, anti-Myc and anti-acetylated H3 antibodies or irrelevant anti-IgG antibody as negative control. PCR primers covering proximal hTERT promoter were used. Leptin (-), untreated cells, Leptin (+), 200 ng/ml for 48 h, Leptin (++), 100 ng/ml for 2 months