Figure 6

mRNA and protein expression of selected interesting genes. (A) qRT-PCR analysis of mRNA expression of SOCS2, CD44, PIK3R1, BMP7, CXCL12 and CD24 was performed on RNA from MCF-7, MCF-7-14 and MDA-MB-231 cells. Results were normalized to internal control GAPDH mRNA and represented relative to mRNA levels of MCF-7 cells. Data are the means ± SD (n = 3). Statistical analysis was performed by ANOVA and Tukey-Kramer multiple comparison.*, P < 0.01, compared with MCF-7 cells. (B) CD44 and CD24 proteins expressed on three human breast cancer cell lines (MCF-7, MCF-7-14 and MDA-MB-231) and single-cell clones derived from MCF-7 and MCF-7-14 cell lines (highly invasive MCF-7-14 CL6 and non-invasive MCF-7 CL17, panel C) were analyzed by flow cytometry. (C) Matrigel invasion assay of single-cell clones derived from MCF-7 (CL17-20) and MCF-7-14 (CL1-8) cell lines. Cells invading Matrigel were stained with crystal violet on the underside of the chamber.