Figure 1

Western blot analysis of rabbit polyclonal antibodies against Argonaute proteins in Hela or 293 cell lines. (A) Total protein extracted from Hela or 293 cells was subjected to Western blot with polyclonal antibodies against EIF2C1-4 proteins. There were the bands at expected molecule mass corresponding to each member of AGO subfamily in the films. (B) Total protein extracted from Hela or 293 cells was subjected to Western blot with polyclonal antibodies against PIWIL1-4 proteins. There were the bands at expected molecule mass corresponding to each member of PIWI subfamily in the films.