Figure 5

Phenotypic effects of HIF siRNA. Effect of HIF siRNA on (a) cell number, (b) apoptosis and (c, d) proliferation under hypoxia in ES and OS cell lines. Bar graph data is normalised to the relevant scrambled siRNA control (white bars): *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant. (c) Proliferation assessed in ES cell lines by Western blot for Ki-67. N, normoxia; H, hypoxia. Numerical values represent mean fold change in Ki-67 as assessed by densitometry:*, p < 0.05; **, p < 0.01 versus hypoxic scrambled (scr) control.