Figure 2
Identification of scFv N14 antigen. SDS-PAGE and Western blot analysis of the nuclear protein extract from HepG2 cells showing two bands of 35 kDa and 37 kDa recognized by scFv N14 antibody (indicated by the double-arrows). Lane 1 = nuclear protein extract. Lanes 2 and 5 = nuclear protein extract incorporating a marker protein (His6-contain protein, MW = 31.0 kDa, indicated by short gray arrow). Lanes 3 and 6 = marker protein alone (long black arrow). Lane 4 = molecular weight standards. The proteins identified in both two bands (band up and band down) were highlighted on the right.