Gene | Forward primer [5'→3'] Reverse sequence [5'→3'] | Anchoring Exons | Amplicon size | Spanning on genome | Amplification efficiency | Reference |
---|---|---|---|---|---|---|
ACTB | CATCGAGCACGGCATCGTCA TAGCACAGCCTGGATAGCAAC | Exon 3 Exon 4 | 211 bp | 652 bp | 1.971 | [28] |
GAPDH | TGCACCACCAACTGCTTA GGATGCAGGGATGATGTTC | Exon 7 Exon 8 | 177 bp | 370 bp | 1.999 | [29] |
HPRT1 | AGACTTTGCTTTCCTTGGTCAG TCAAGGGCATATCCTACAACAA | Exon 6 Exon 8 | 151 bp | 5120 bp | 1.949 | [30] |
B2M | ACTGAATTCACCCCCACTGA CCTCCATGATGCTGCTTACA | Exon 2 Exon 4 | 114 bp | 741 bp | 1.924 | [28] |
18S rRNA | GTAACCCGTTGAACCCCATT CCATCCAATCGGTAGTAGCG | NA1 | 151 bp | 151 bp | 2.000 | [31] |
RPL29 | GGCGTTGTTGACCCTATTTC GTGTGTGGTGTGGTTCTTGG | Exon 1 Exon 2 | 120 bp | 507 bp | 1.937 | [16] |
GPNMB | TGCGTCCGTGAGAATTCA TGTGCTCCCTCATGTAAGCA | Exon 1 Exon 2 | 144 bp | 6522 bp | 1.945 | In house design2 |