Figure 1
From: Estrogen regulation of TRPM8 expression in breast cancer cells
Analysis of TRPM8 mRNA and protein expression in MCF-7 cells. (A) RT-PCR analysis of human TRPM8 transcripts expression in MCF-7 cells on three independent cell lysates. Human colon extract was used as positive control for the detection of TRPM8. (B) Western blot analysis of TRPM8 proteins were performed on total lysate (lanes 1-2) of two independent cell extracts, or after immunoprecipitation experiment (lane 3). The arrow indicates a TRPM8 band, and (*) a non specific band.