Figure 4

Effect of CARM1 in androgen-stimulated androgen receptor mediated responses. A-D, Androgen receptor (AR)-negative CV-1 cells, AR-positive LNCaP cells, and androgen-independent C42 and CWR22RV cells were transiently transfected with 100 ng of pGL-ARE4-luc, various amounts of pSG-CARM1, and 2 ng of renilla with or without 10 nM R1881. When needed, the pARO vector was cotransfected as indicated. E-F, the PSA promoter (p61-luc) was used, instead of pARE4-luc. Luciferase assays were performed 48 h post-transfection. Relative luciferase activity (RLU) was measured, and the hormone-treated group was normalized by the non-hormone-treated group. Values are represented as fold change. Each bar represents the mean ± S.D.