Figure 7
THL inhibits hypoxia-induced HIF-1α and VEGF-A expression in MDA-MB-231 breast cancer cells. MDA-MB-231 cells were cultured under normoxia or hypoxia (with 200 μM CoCl2, a hypoxia-mimetic agent, in the medium) in serum-free medium containing various concentrations of THL for 24 h. Protein level of HIF-1α in cell lysates was analyzed by Western blot A), and VEGF-A secreted into the conditioned medium was analyzed by ELISA B). For the secreted VEGF-A, values represent means ± SD, n = 4. **P < 0.001 versus untreated cells under hypoxia. The conditioned media from untreated and THL-treated cells under hypoxia were tested for their effect on HMEC-1 tube formation C).