Table 2 Primers used for PCR and sequencing.
From: Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma
| Â | forward primer 5'-3' | reverse primer 5'-3' | bp | Ref-sequ. |
|---|---|---|---|---|
SDHA fragment 1* | GCGAAGGACCTGGCGTCTA | CCCAGAGTGCAGAAGCGTAT | 1100 | NM_004168.2 |
SDHA fragment 2 | GACTGCGCGGCGGCAACA | CAGCCTCTTCCTTCTCGGAT | 1094 | NM_004168.2 |
SDHB Exon 1 | GGGTCCTCAGTGGATGTAGGCT | GCCTTGCCCTATGCTTCCTC | 272 | NM_003000.2 |
SDHB Exon 2 | GAATGCCTGCCTTTTCTAAGAAGA | GCCATCGGATGATCTCAGATTTT | 377 | NM_003000.2 |
SDHB Exon 3 | CCTGAGAAGACCAAATGGATAAGC | AGCTGCAGCTGTTTTCCAGATG | 300 | NM_003000.2 |
SDHB Exon 4 | TGTTGCATGTCAGTGCTGCCC | GGTCCTCCTCCTGCCATAATATAGG | 423 | NM_003000.2 |
SDHB Exon 5 | CGAGTAGTCAGTGTCCAAGAAATGG | AATGGCTTGCATCAGCTTATGTTC | 317 | NM_003000.2 |
SDHB Exon 6 | TTTGTTCATGCACTGACCCCA | AAACCAGGCCCCTCAGAATG | 307 | NM_003000.2 |
SDHB Exon 7 | TGAATTCCCTTTCCTCTGCACTC | ATGACTAGGGTTGCTCTCTGCCA | 323 | NM_003000.2 |
SDHB Exon 8 | TTGCTTGGACACTGAACCAGCT | GCTGTATTCATGGAAAACCAAGATC | 286 | NM_003000.2 |
SDHC Exon 1 | GCGTCACTTCCGTCCAGACC | CTGCCCAGGCACAGGATAAA | 150 | NM_003001..3 |
SDHC Exon 2 | CCCTTCACCCCTAAAAATAGAGAAG | AAAATAATCTCCAGGGCCGGG | 480 | NM_003001..3 |
SDHC Exon 3 | CCTGGCTTGGTATTGCAAAATATTG | AAGGGTTCACCTCATCTACATAGCA | 336 | NM_003001..3 |
SDHC Exon 4 | ACCTATTCAGGAGAATTGCTTGGA | ATCAAGTGCTGAGTTTCAAAGGAGG | 382 | NM_003001..3 |
SDHC Exon 5 | TGGTTTAGAATTGTATGAGGTGCCA | GCGAGACTCCACTCTTGGGAA | 387 | NM_003001..3 |
SDHC Exon 6 | CGCTTTTCTCTAGAATCATGCTGAG | TCTACTGCTCCAAGGAGATCTGAAA | 375 | NM_003001..3 |
SDHD Exon 1 | CCAGCATTTCCTCTTCCCTGTT | CTCCTCAAGAGATCTCCCACCC | 252 | NM_003002.1 |
SDHD Exon 2 | TGGTCTTAACTTCACAGTAACCCCA | GGTCTTCATTTGACAAAGTTGGACA | 413 | NM_003002.1 |
SDHD Exon 3 | TGTACACTGCCTGTCAGTTTGGG | TAGGGCATTTCAATCAACTTCTCCC | 345 | NM_003002.1 |
SDHD Exon 4 | GGAGACATTGCATTTGAACTTGACA | AAAGCAGAGGCAAAGAGGCATACAT | 334 | NM_003002.1 |
LYRM4 | TCGTACTTGGGACCTCGGCGAA | TGGATGCTGAAGGTGGTCCCTG | 379 | NM_020408.3 |
Nfs1 fragment 1 | TGCTCCTCAGTCTGCGGTT | CCCCTTTGGGACCGTAGATT | 686 | NM_021100 |
Nfs1 fragment 2 | TCCATACTGATGCAGCCCA | CCAATCTAGAGCATCCACTAGG | 789 | NM_021100 |
Frataxin fragment 1 | ACATCGATGCGACCTGCAC | TGACACATAGCCCAACTGTCC | 638 | NM_181425.1 |
Frataxin fragment 2 | GCAGAGGAAACGCTGGACT | TGACACATAGCCCAACTGTCC | 478 | NM_181425.1 |
ALK fragment 1 | GCTGGGTACCAAGGACTGTTCA | CTGGTTCCTGAGGTCATGCAGT | 957 | CCDS37172.1 |
ALK fragment 2 | AAGACTCCTTCCCTTTCCTGTCTC | AAGGACACGTTTCCCCTCAAGA | 949 | CCDS37172.1 |
ALK fragment 3 | GTTCCAGGACCACCAAGACCAT | AAATACGTAGGTGGCTCCACCC | 955 | CCDS37172.1 |
ALK fragment 4 | CCTGCCCCAGTACAAACCAGTT | GCAGTAGTTGGGGTTGTAGTCGGT | 947 | CCDS37172.1 |
ALK fragment 5 | CTGGCTTTCTCCGGCATCAT | CCTGTCTTCAGGCTGATGTTGC | 972 | CCDS37172.1 |
ALK fragment 6 | CTTGGATATATGCCATACCCCAGC | GTTTGTGAAGGAGCCATTGCCT | 970 | CCDS37172.1 |
PHOX2B Exon1 | CTCCAGCCACCTTCTCCATA | TTCCTATATACGGGCGGAAA | 369 | NC_000004.11 |
PHOX2B Exon2 | CAGCTTCTCTCGGCAACTCT | GGCCCTAGGTCCTTCTCACT | 426 | NC_000004.11 |
PHOX2B Exon3 | ACCCTAACCGGTGCTTTTCT | ACAATAGCCTTGGGCCTACC | 686 | NC_000004.11 |
SDHAF1 | CTGAGCGTCTCTGCTTAGCC | CTCTGACATCCCCAATTCGT | 506 | NM_001042631.1 |
SDH5-fragment 1 | GTTTCCGGTGCAGGTGGG | GCACACAGTAGGCTCACCAA | 251 | NM_017841.1 |
SDH5-fragment 2 | CCTGGCCACAGTGTAATTT | TCAAATCAGCCTAAACTGTCCT | 675 | NM_017841.1 |
SDH5-fragment 3 | GTGGTTCTTGGCCAGTGTTT | GCAAGGCTAACGTCCATCAT | 297 | NM_017841.1 |