Figure 5
From: Rac1 and Cdc42 are regulators of HRasV12-transformation and angiogenic factors in human fibroblasts
Activated Rac1 and Cdc42 independently regulate uPA expression. The indicated cell strains were serum starved for 24 hours then stimulated with medium containing 10% SCS. (a) Grown in the absence of tetracycline, PH3MT cell strains expressing a vector control (PH3MT-VC-C2), Rac1N17(PH3MT- Rac1N17-C1), Cdc42N17 (PH3MT-Cdc42N17-C2) or both Rac1N17 and Cdc42N17 (PH3MT-Rac1N17/Cdc42N17) were tested for uPA expression levels using ELISA. Data is presented as percent of control. Error bars indicate the SD from triplicate experiments. * indicates significant difference, p < 0.05. ** indicates a significant difference compared to PH3MT-Rac1N17-C1 and PH3MT-Cdc42N17-C2, p < 0.05. (b) conditioned medium was collected from MSU-1.1 cells expressing GFP alone (MSU-1.1-GFP-VC), GFP-tagged Rac1V12 (MSU-1.1-GFP-Rac1V12) or GFP-tagged Cdc42V12 (MSU-1.1-GFP-Cdc42V12), and uPA expression was analyzed. Data is presented as fold-induction of uPA expression. Error bars represent the SD from triplicate experiments. * indicates significant difference, p < 0.05.