Figure 3
From: Effects of clusterin over-expression on metastatic progression and therapy in breast cancer
Effect of TNFalpha on p21 and Bax in MCF-7 and MCF-7CLU cells. Panel A: Nuclear extracts prepared from MCF-7 and MCF-7CLU cells treated with ethanol or 5 ng/ml TNFalpha for 48 h were separated on 10% SDS-PAGE gels and immunoblotted with mouse monoclonal antibodies against p21. Nuclear p21 protein expression was normalized against lamin A/C and plotted relative to appropriate control. Blots are representative of two independent experiments. Panel B: Cytosolic fractions prepared from MCF-7 and MCF-7CLU cells treated with ethanol vehicle or 5 ng/ml TNFalpha for 48 h were separated on 12.5% SDS-PAGE gels and immunoblotted with monoclonal antibodies directed against bax and GAPDH. Non-nuclear membrane (NNMF) fractions were separated on 12.5% SDS-PAGE gels and immunoblotted with monoclonal antibodies directed against Bax, GAPDH and ATP synthase. Blots are representative of two independent experiments. Data are expressed as mean ± range.