Table 1 Primary antibodies used in the study.
| Â | Â | Â | Â | Antibody dilution | ||
|---|---|---|---|---|---|---|
Targeted antigen | Clone name | Manufacturer | Ab type | aFC | bpIHC | Confocal imaging |
Primary antibodies | ||||||
Ep-CAM | HEA-125 | Miltenyi-Biotec SAS | FITC-mouse IgG1 | 1:11 | - | 1:50 |
CK20 | IT-Ks 20.10 | Biovalley | FITC-mouse IgG1 | 1:50 | - | 1:50 |
Beta-Catenin | β-catenin-1 | DAKO | mouse IgG1 | 1:20 | - | 1:50 |
Beta-Catenin | 14/Beta-Catenin | BD Biosciences | mouse IgG1 | - | 1:400 | - |
CA 19-9 | C241:5:4:1 | Novacastra | mouse IgG1 | 1:20 | 1:200 | 1:50 |
CD133 | AC133 | Miltenyi-Biotec SAS | mouse IgG1 | 1:11 | - | 1:50 |
EBP50 | - | Calbiochem | rabbitcpAb | - | - | 1:300 |
CD44 | G44-26 | BD Biosciences | mouse IgG2b | 1:50 | 1:10 | 1:50 |
ALDH1 | 44/ALDH | BD Biosciences | mouse IgG1 | - | 1:100 | 1:100 |