IL-6 signaling by STAT3 participates in the change from hyperplasia to neoplasia in NRP-152 and NRP-154 rat prostatic epithelial cells

Table 1 Inhibition of JAK2 resulted in apoptosis of NRP-152 cells

Cell	Rx	μM	% Apoptotic +/- SD
NRP-154	AG490	0	8 + 4
(uncloned)		30	8.5 + 5
		100	7.5 + 5
154 clone A3	AG490	0	7.2 + 2
(high IL-6)		100	9.8 + 3
154 clone B1	AG490	0	8.5 + 5
(high IL-6)		100	9.3 + 4
154 clone G3	AG490	0	3.7 + 3
(low IL-6)		100	8.3 + 5
154 clone G7	AG490	0	13.2 + 5
(low IL-6)		100	11.9 + 7
NRP-152	AG490	0	7.5 + 4
		30	12 + 0
		100	45 + 10^*

Legend: NRP-152 and NRP-154 cells were placed in 60 mm plates for 48 hr with compound at the concentrations indicated. Zero concentration of the compound is the vehicle (DMSO) control. At the end of the incubation period, cells were harvested, washed, and stained with FITC-annexin V, to demonstrate apoptotic cells. Quantification of fluorescence was performed on a Becton-Dickinson FACScan flow cytometer using CellQuest software. ^* p < 0.005 by Student t-test, compared to vehicle-treated cells.

ISSN: 1471-2407