Figure 4

Curcumin suppresses the expression level of FABP5 and PPARβ /δ in MDA-MB-231 cells. (A) MDA-MB-231 cells were treated with varying concentration of curcumin for 24 hours (0.1% DMSO was used for the control) prior to cell lysis. Cell lysates were resolved with SDS-PAGE and immunoblotted with antibodies recognizing FABP5, and β-tubulin was used as a loading control (top panel). Bottom panel: Densitometry analyses provide the relative amount of FABP5 normalized to β-tubulin. The analysis was performed in triplicates as mean of ± SE. +, p = 0.04 versus control, + +, p = 0.03 versus control. (B) Total RNA was collected from MDA-MB-231 cells treated with curcumin (30 μM) for 4 hours or 0.1% DMSO as control. Expression level of FABP5 was analyzed by qRT-PCR. GAPDH was used for normalization. Data are mean of ± SE (n = 3). * p = 0.0002 versus control treatment (C) MDA-MB-231 cells were treated with varying concentration of curcumin for 24 hours (0.1% DMSO was used for the control) prior to cell lysis. Cell lysates were resolved with SDS-PAGE and immunoblotted with antibodies recognizing PPARβ/δ, and β-actin was used as a loading control (top panel). Bottom panel: Densitometry analyses provide the relative amount of PPARβ/δ normalized to β-actin. The analysis was performed in triplicates as mean of ± SE. +, p = 0.04 versus control, + +, p = 0.02 versus control (D) Total RNA was collected from MDA-MB-231 cells treated with curcumin (30 μM) for 4 hours or 0.1% DMSO as control. Expression level of PPARβ/δ was analyzed by qRT-PCR. GAPDH was used for normalization. Data are mean of ± SE (n = 3)** p = 0.0003 versus control treatment.