Figure 4

Measurement of caspase activity after cell treatment with curcumin- and emodin-derived inhibitors. Curcumin- and emodin-derived inhibitors of CSN-associated kinases induce apoptosis in B8 cells. Caspase-3/7 activities in mouse B8 fibroblasts were determined using a DEVD-peptide. Cells (10⁴/well) were treated with the inhibitors curcumin, piceatannol, BTB14431, SEW04213 and JFD02836 for 4 h at 37°C. After incubating cells with caspase-3/7 reagent for 3 h at room temperature the fluorescence (RFLU: relative fluorescent light units) was measured at an excitation wavelength of 485 nm and an emission wavelength of 530 nm.