Prognostic factors and treatment strategy for metastatic myxoid liposarcoma

Myxoid liposarcoma (MLS) has the tendency to metastasize extrapulmonary. Although prognostic factors at initial diagnosis of MLS have been reported, those at diagnosis of metastasis remain unclear. The purpose of this study was to investigate the prognostic factors for disease-specific survival at the initial diagnosis of metastasis. This retrospective observational study was conducted at three cancer centers and two university hospitals in Japan. Of 274 MLS patients pathologically diagnosed between 2001 and 2015, 48 metastatic patients were examined.


Introduction
Myxoid liposarcoma (MLS) is the second most common type of liposarcoma, representing approximately one third of all liposarcomas and 10% of all adult soft tissue sarcomas [1,8]. MLS carries an intermediate risk with approximately one third of patients developing metastases and eventually dying of these tumors [5,11,14,23].
Factors found to influence the prognosis at initial diagnosis of MLS include patient age, tumor size, tumor depth, the surgical margins achieved, and morphological factors such as grading, necrosis and mitotic rate, proliferation index (MiB-1, Ki-67 immunostain), and P53 overexpression [5,9,11,14]. Of these, the amount of round cell component is reported to be the most important factor affecting the development of distant metastases or survival [2,9,12,14,16].
MLS has been reported to have a characteristic metastatic behavior; the incidence of extrapulmonary metastases, including the trunk and extremities, is as high as 87% of the metastatic MLS patients [10]. Although some studies have investigated the metastatic patterns of MLS, the prognostic factors related to metastasis characteristics at the initial diagnosis of metastasis remain unclear. Spillane et al. [24] have recommended aggressive treatment for metastatic disease involving further surgery; however, a treatment strategy for metastatic MLS has yet to be established.
In this retrospective, multi-center study, we enrolled MLS patients with metastasis, examined the characteristics of the metastases, and investigated the prognostic factors affecting overall survival at the initial diagnosis of metastasis. Then, we discuss a treatment strategy for metastatic MLS. follow-up. Basically, whole body CT was performed at least once a year until 5 years after the initial diagnosis. But 2 patients were followed by plain chest radiography only, and 5 were followed at other hospitals.
We extracted the data from a total of 52 (19.0%) patients with metastasis; however, we excluded four patients whom we could not follow up after the diagnosis of metastasis.

Data collection
We extracted the following clinic-pathological data collected at the time of initial metastasis diagnosis from the database: age; sex; time between the diagnosis of primary lesion and metastasis; number of metastases; location of each metastasis; diameter of the largest metastasis; and modality used for detecting metastasis. In addition, we examined the treatment details for the metastasis. We defined local radical treatment as R0 resection, R0 or R1 resection with adjuvant radiation therapy, or radiation therapy with ≥ 60 Gy, including carbon ion therapy and photon beam therapy, and semi-radical treatment as radiation therapy with ≥ 40 Gy. We next defined status after local treatment; no evidence of disease (NED) signified that no detectable disease remained following radical or semi-radical treatment of all metastases, while alive with disease (AWD) signified any remaining lesion, regardless of local treatment. In addition, we examined the prognosis at final follow-up (NED, AWD, or dead of disease [DOD]). Finally, we analyzed the prognostic factors for overall survival following the initial diagnosis of metastasis.
Regarding primary tumor, we also extracted the following data: age at diagnosis of primary tumor, location, size, if radical treatment or adjuvant chemotherapy were done.
For NED patients, we also examined if the patients received adjuvant chemotherapy.

Statistical analysis
The duration from primary diagnosis to metastasis and cumulative survival rates were calculated using the Kaplan-Meier method. The difference in time to metastasis by existence of lung metastases was analyzed using the log-rank test. Survival following diagnosis of metastasis was defined as the time to disease specific death. Prognostic factors were identified by Cox-proportional hazards regression analysis. A p-value < 0.05 was considered statistically significant. Variables revealed to be significant by univariate analysis were evaluated by log-rank test and multivariable analysis using Cox-proportional hazards model. Statistical analyses were performed with JMP 13 (SAS Institute Inc., Cary, NC, USA).
Factors for achieving NED were analyzed by logistic regression analysis. Factors revealed to be significant by univariate analysis were examined using multivariable analysis.

Results
Median follow-up following primary diagnosis was 47.4 (4.7-187.2) months and that following initial metastasis was 25.8 (2.1-96.8) months. The baseline clinical characteristics at initial diagnosis of metastasis are shown in Table 1 The metastasis treatment characteristics and outcomes are shown in Table 2. Twentyeight patients were undergoing local radical treatment, 23 had undergone resection, and five had undergone radical radiotherapy with ≥ 60 Gy including carbon ion and photon beam therapy. Two patients had undergone semi-radical radiotherapy with ≥ 40 Gy. As a result of local radical or semi-radical treatment, 26 patients had become NED. Six patients did not become NED after local treatment, as the resection had been intralesional or new metastases were detected soon after treatment. Among 26 NED patients, 9 patients The median disease-specific survival following the diagnosis of metastasis was 52.5 months and the 5-year survival rate was 40.6% ( Fig. 2A). The median disease specific survival following the diagnosis of the primary tumor for metastatic patients was 87.3 months and the 5-year survival rate was 56.2% (Fig. 2B).
Next, we analyzed the prognostic factors at the initial diagnosis of metastasis (Table 3).
Univariate analysis indicated that time to metastasis (p = 0.019) and liver metastasis (p = 0.005) were significantly related to worse prognosis. NED following local treatment was significantly related to better prognosis (p = 0.0007). Moreover, the survival curve examined using the Kaplan-Meier method showed a significant difference in survival for these three factors (Fig. 3A-C). Based on multivariable analysis, disease specific survival  Table. 2).
Subsequently, we analyzed the factors related to NED achievement (Table 4)

Discussion
The Prognostic factors of MLS at the initial diagnosis have been described in previous studies. However, to date, the prognostic factors for metastatic MLS patients have not been elucidated in detail. In this retrospective study, we analyzed 48 metastatic MLS patients, examined the clinical course, and investigated the prognostic factors at the initial diagnosis of metastasis. Multivariable analysis indicated that liver metastases and NED achievement following radical or semi-radical local treatment for metastasis, including resection and radiation, were significant factors for longer survival. Number of metastases and abdominal/retroperitoneal metastases were the significant inhibitory factors for achieving NED. To the best of our knowledge, this is the first study to statistically demonstrate the importance of achieving NED for longer survival in metastatic MLS patients.
We first examined the epidemiology of the metastases and found that extrapulmonary metastasis was detected in 93.8% of metastatic MLS patients, which is as high as proportion reported to date. Previous reports have shown MLS metastasizes to extrapulmonary sites, including the abdominal wall and cavity, retroperitoneum, subcutaneous soft tissue, and bone, at a rate as high as 86.5% in metastatic patients [1,3,8,10,15,25]. There has been no consensus about the mechanism why MLS tends to metastasize extrapulmonary. Asano et al. [3] have suggested that large tumor size and low histological grade are significantly associated with extrapulmonary metastasis; however, Haniball et al. [10] have reported that there is no clear correlation between the site of first metastases, tumor size, or the round cell component of the primary tumors.
Our data indicates that lung metastases appear significantly earlier than extrapulmonary metastases (Fig. 1B), indicating that the mechanism of metastasis differs between pulmonary and extrapulmonary metastases or that MLS which metastasize to the lung are more aggressive than those that do not. Further investigation is needed to elucidate the mechanisms underlying the tendency of MLS to metastasize extrapulmonary, as well as the differences between pulmonary and extrapulmonary metastases, to ensure proper management of MLS metastases.
In the present study, the liver was the only significant metastatic site for poor prognosis in univariate and multivariable analyses and the survival tended to be shorter in patients with pulmonary metastases in univariate analysis (p = 0.09). Previous reports have shown that the disease-free interval and the overall survival rate were significantly better in patients with extrapulmonary metastases compared to those with pulmonary metastases [3,6,17]. Liver metastasis has not been reported to be important for survival, as these previous studies did not analyze the prognosis by each metastatic organ. Taken together, our current results and these previous reports suggest that liver and lung metastases may be prognostic factors of shorter survival. As vital organ such as brain, lung, or liver metastasis is associated with poor prognosis in common cancers [13], it is plausible that lung and liver metastasis may also be related to poor prognosis in MLS patients. In addition, the poor prognosis of patients with lung metastases might be due to more aggressive biology or the speed, as discussed in the previous paragraph.
In this study, NED achievement was one of the significant factors related to disease specific survival in multivariable analysis. A number of reports support our findings.

Spillane et al. [24] analyzed the natural history of soft tissue metastasis from MLS and
concluded that soft tissue metastases should be managed aggressively, most often involving further surgery, because patients only with soft tissue metastasis have relatively long prognosis. There are two case reports of long-term survival following complete resection of metastases [22,26]. Although extrapulmonary metastasis has been thought to be equivalent to systemic metastasis in all types of sarcomas, long-term survival has been achieved when a complete resection was possible for both the pulmonary and extrapulmonary metastases [4]. Our results and these reports indicate that complete resection of metastases from MLS should be considered, if possible.
We had hypothesized that complete resection of metastases might lead to the best prognosis; however, NED achievement by resection was not significant in the present study (data not shown). As MLS is known to be particularly sensitive to radiotherapy compared with other histological subtypes of soft tissue sarcoma [7,18], we included patients who were administered radical and semi-radical radiotherapy for the metastasis, which significantly improved the prognosis of NED patients. In addition to patients who underwent radical radiation therapy, including carbon ion therapy, photon beam therapy, and conventional radiation therapy with ≥ 60 Gy, there were two patients who achieved NED by semi-radical radiation therapy with ≥ 40 Gy. In both cases there was no local recurrence surrounding the irradiated metastasis at the final follow up, indicating that semi-radical radiation therapy might be useful for extending survival when radical therapy cannot be adapted. Based on these data, the first choice treatment strategy should be metastasis resection; however, if this is not possible, radiotherapy might be the second choice, as this could lead to a better prognosis. Further investigation is needed with a larger number of patients to determine the effect of radiotherapy on survival.
The number of metastases and metastases to abdomen or retroperitoneum were the significant inhibitory factor for achieving NED. As the number was significant factor, it might be better to detect metastases while the number is low by periodic surveillance.
Total body MRI has been proposed for early detection of bone and soft tissue metastases, including MLS metastasis [19,21]. However, total body MRI is not common in Japan or around the world. Importantly, both PET scans and bone scans are reported as not highly sensitive to MLS metastases [20,21]. In the present study, the metastases of 35 (73.1%) patients were detected by CT. As CT is one of the most common imaging modalities, liver metastasis was one of the worse prognostic factors by multivariable analysis, and abdominal/retroperitoneal metastases were factors inhibiting the achievement of NED, contrast-enhanced CT might be useful for metastasis surveillance in MLS patients. In the present study, the number of patients who developed metastasis linearly increased to 80% within the first 4 years and approximately 90% of the metastatic patients were diagnosed within 8 years (Fig. 1A). Thus, we recommend whole body surveillance 2-3 times per year during the first 4 years and once a year till 8 years. As the presence of a round cell component on histopathology was reported to be predictive of a much higher metastatic rate compared with the absence of a round cell component [14,23,24], surveillance should be recommended especially for patients with a round cell component > 5%. It should also be noted that a number of patients will develop metastases beyond the 8 years after the initial diagnosis. As it is not pragmatic to survey all patients with whole body CT once a year after 8 years of follow-up, we should carefully listen to patients over 15 years, check whether there are symptoms or signs of recurrence, and perform contrastenhanced CT if any appear.
This study had some limitations. First and most importantly, this was a retrospective 13 study. Although we showed that achievement of NED was the important prognostic factor in multivariable analysis, it is possible that the tumors of NED patients might have been less aggressive originally. Second, we did not investigate the proportion of the round cell component. Although previous study which analyzed 160 patients reported that survival following the diagnosis of metastasis was not affected by the round cell component [10], it may be still important and has to be confirmed in the future. Third, we did not examine the effect of palliative chemotherapy. In this study, as we investigated the prognostic factors at the initial diagnosis of metastasis, palliative chemotherapy was performed only for patients without NED. Because all of the patients with metastasis including NED patients at the initial diagnosis would receive palliative chemotherapy when metastases get locally uncontrollable, we thought we would not be able to see the effect. However, trabectedin, the effect of which is apparent for MLS, was not used in daily practice because it had not been covered by insurance in Japan during the study registration period.

Conclusions
Over 90% of MLS metastatic patients had extrapulmonary metastases. Although only 18.8% had lung metastasis, it appeared significantly earlier than extrapulmonary metastases. As multivariable analysis indicated that NED achievement following resection or radiation therapy were significant factors for longer survival, resection or radiation therapy should be considered for all of the metastases. As number of metastases was a significant factor for achieving NED, early detection of metastases might be important.