Development of a highly metastatic model that reveals a crucial role of fibronectin in lung cancer cell migration and invasion

Background The formation of metastasis is the most common cause of death in patients with lung cancer. A major implement to understand the molecular mechanisms involved in lung cancer metastasis has been the lack of suitable models to address it. In this study, we aimed at establishing a highly metastatic model of human lung cancer and characterizing its metastatic properties and underlying mechanisms. Methods The human lung adeno-carcinoma SPC-A-1 cell line was used as parental cells for developing of highly metastatic cells by in vivo selection in NOD/SCID mice. After three rounds of selection, a new SPC-A-1sci cell line was established from pulmonary metastatic lesions. Subsequently, the metastatic properties of this cell line were analyzed, including optical imaging of in vivo metastasis, immunofluorescence and immunohistochemical analysis of several epithelial mesenchymal transition (EMT) makers and trans-well migration and invasion assays. Finally, the functional roles of fibronectin in the invasive and metastatic potentials of SPC-A-1sci cells were determined by shRNA analysis. Results A spontaneously pulmonary metastatic model of human lung adeno-carcinoma was established in NOD/SCID mice, from which a new lung cancer cell line, designated SPC-A-1sci, was isolated. Initially, the highly metastatic behavior of this cell line was validated by optical imaging in mice models. Further analyses showed that this cell line exhibit phenotypic and molecular alterations consistent with EMT. Compared with its parent cell line SPC-A-1, SPC-A-1sci was more aggressive in vitro, including increased potentials for cell spreading, migration and invasion. Importantly, fibronectin, a mesenchymal maker of EMT, was found to be highly expressed in SPC-A-1sci cells and down-regulation of it can decrease the in vitro and in vivo metastatic abilities of this cell line. Conclusions We have successfully established a new human lung cancer cell line with highly metastatic potentials, which is subject to EMT and possibly mediated by increased fibronectin expression. This cell line and its reproducible s.c. mouse model can further be used to identify underlying mechanisms of lung cancer metastasis.


Background
According to the World Health Organization, lung cancer is responsible for more than 1.3 billion deaths worldwide annually. Despite advances in the treatment of primary tumours, recurrence and metastasis are the most common cause of death in patients with lung cancer. The current poor understanding of the molecular mechanisms involved in lung cancer metastasis is due, in large part, to the lack of suitable models for its study [1,2]. Although many metastatic models have been successfully used to identify molecular elements during metastasis, most rely on the introduction of tumour cells directly into the systemic circulation. These models do not represent the steps of detachment of tumour cells from the primary tumour--invasion and intravasation--and therefore are unlikely to reveal genes involved in these early steps of metastasis [3,4]. A metastatic model that can represent the full spectrum of metastasis is rare, especially for lung cancer, so it is necessary to develop a spontaneously met-astatic model of human lung cancer, so as to provide a platform for uncovering the underlying mechanisms.
EMT, a process whereby cells acquire molecular alterations that facilitate cell motility and invasion, has been shown to play an important role in tumour metastasis [5]. More recently, there are also observations suggesting that the EMT program exists in lung cancer and correlates with the poor prognosis of patients with lung cancer [6,7]. However, these works are mostly based on cultured cell models, and the precise roles of EMT in lung cancer metastasis are still largely unclear. Metastases ultimately develop in secondary organ sites as a consequence of the interactions between tumour cells and the host microenvironment [8]. Fibronectin, a glycoprotein in extracellular matrix and also a mesenchymal maker of EMT, has been implicated in the development of multiple types of human cancer [9,10]. In lung cancer, fibronectin expression is increased and has been implicated in promoting lung cancer growth and conferring resistance to therapy [11,12]. In addition, fibronectin has been shown to promote lung cancer cell migration and invasion by increasing MMP-9 expression or activating FAK signaling [13,14]. However, the specific role and molecular basis of fibronectin in lung cancer metastasis are still elusive.
In the present report, we successfully develop a spontaneously metastatic model of human lung cancer that represents the full spectrum of metastasis, from which a highly metastatic human lung cancer cell line, termed SPC-A-1sci, was derived. This cell line exhibits typical changes in cellular phenotype similar to EMT. Moreover, fibronectin plays an important role in these alterations, and thus resulting in the highly metastatic potentials of this cell line.

Cell lines and cell culture
The human lung adeno-carcinoma cell line SPC-A-1 was obtained from Cellular Institute of Chinese Academy of Science (Shanghai, China). This cell line was originally isolated from the surgical specimens of a Chinese man with advanced lung adeno-carcinoma by Shanghai Chest Hospital and Cellular Institute of Chinese Academy of Science in 1980 [15]. The cells were cultured at 37°C under a 5% CO 2 atmosphere in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, UT), 100 U/ml penicillin, and 100 μg/ml streptomycin. Cells were regularly certified as free of Mycoplasma contamination.

Animal experiments
Five-to 6-week-old male congenitally immune-deficient nonobese diabetic/severe combined immune-deficient (NOD/SCID) mice were maintained under specific pathogen-free (SPF) conditions. Mice were manipulated and housed according to protocols approved by the Shanghai Medical Experimental Animal Care Commission. To isolate a highly metastatic cell line, briefly, 2.0 × 10 6 of the SPC-A-1 cells were injected subcutaneously (s.c) into NOD/SCID mice. When the subcutaneous tumour developed, small pieces of tumour tissue were implanted into the s.c. sites of mice in the first generation of mouse models and the primary tumours were excised 4 weeks later. Those mice were sacrificed under deep anesthesia when they showed signs of distress, and visual lung metastases were isolated and s.c. implanted into the new recipient mice in the second generation of mouse models for in vivo selection. These procedures (lung metastasis, s.c. implantation, lung metastasis) were repeated for three rounds. At the end of the selection, the lungs harboring massive metastatic lesions were isolated and s.c. implanted into new recipient mice, after which the primary tumour was removed to initiate in vitro primary culture.
Subcutaneous tumour implantation was performed as described [15]. In brief, an SPC-A-1 flank-grown tumour was removed from a NOD/SCID mouse and was rinsed and minced in cold phosphate-buffered saline (PBS). The tumour was implanted by the s.c. injection of minced tumour tissue suspended in PBS using a 1 cc tuberculin syringe and an 18-gauge needle.
For primary tumour growth assays and spontaneous metastasis via s.c. injection, cells (2 × 10 6 per mouse) were injected subcutaneously into the right upper flank region of NOD/SCID mice. Mice were monitored weekly for tumour size and evidence of morbidity related to the primary tumour or metastases. Tumour size was quantified in two dimensions using calipers. Tumour volume was calculated as follows: tumour volume (mm 3 ) = L × W × W/2, where L represents length and W represents width. Nine weeks later, all mice were sacrificed, and the organs, including lungs and livers, were removed and processed for standard histological studies. For histological analysis, the primary tumours and mouse organs were harvested at necropsy and fixed in 10% formalin. The fixed samples were then embedded in paraffin, and three non-sequential serial sections were obtained per animal. The sections were stained with H&E and analyzed for the presence of metastases.

Transduction of tumour cells
The GFP-Luc lentiviral vector encoding a fusion gene of GFP and luciferase was generated by inserting the GFP-Luc gene from the plasmid eGFP-2A-CBGr99 (kindly provided by Professor Hammerling) into the BamHI/ XhoI sites of a pWPXL vector (Addgene). Transductions of SPC-A-1sci and SPC-A-1 cells were performed with the aforementioned lentiviral vector according to instructions supplied by Addgene http://www.addgene.org and stable transfectants were further isolated by cell sorting (Epics Altra, Beckman Coulter) on the basis of their EGFP expression.

Luciferase imaging and GFP imaging
We used a Berthold LB983 NightOwl System (EG&G Berthold, Bad Wildbad, Germany) to monitor the primary tumour growth and distant metastasis of SPC-A-1sci and SPC-A-1 cells in mouse models as previously described [17,18]. For in vivo bioluminescence imaging (in vivo BLI), the animals were injected i.p. with 150 mg luciferin (Luciferin-EF, Promega) per kg of body weight, anesthetized with pentobarbital (10 mg/ml) in sterile water, and then placed in the NightOwl LB 983 Molecular Light Imager. For ex vivo biofluorescence imaging (ex vivo BFI), mice lungs were excised after in vivo BLI and placed in the chamber of the NightOwl LB 983 Molecular Light Imager and imaged.

Immunofluorescence and immunohistochemical analysis
The experiments were performed as described previously [19]. For indirect immunofluorescence analysis, cells were plated and grown on glass slides for 18~20 hours and fixed with 4% paraformaldehyde. The slides were then blocked and incubated with the following primary antibodies: Anti-E-cadherin and ZO-1 were obtained from Santa Cruz (1:50), anti-Vimentin from DAKO (1:100), anti-Fibronectin from Abcam (1:100) and anti-αtubulin and DAPI were obtained from Sigma (1:100). Finally, the slides were incubated with fluorescence con-jugated secondary antibody (Sigma) and viewed with a Fluoview FV1000 microscope (Olympus, Japan).
For immunohistochemical analysis, all tissue samples were fixed in phosphate-buffered neutral formalin, embedded in paraffin, and cut into 5-μm-thick serial sections. Immunohistochemical staining with antibodies to E-cadherin (1:50, Santa Cruz), Vimentin (1:25, DAKO) was performed according to standard procedures. Results were observed and photographed with an Axioskop 2 microscope (Carl Zeiss, Oberkochen, Germany) and DP70 Imaging system (Olympus, Japan).

Soft agar colony formation assay
Colony formation in soft agar was assayed as described previously [20]. Briefly, SPC-A-1sci or SPC-A-1 cells (1000 cells/well) were seeded in 0.3% agar (Sigma) containing culture medium layered on 0.5% agar medium (0.3 ml/well) in 24-well plates and cultured at 37°C under 5% CO 2 for three weeks. The colonies formed were photographed with an Axioskop 2 microscope (Carl Zeiss, Oberkochen, Germany) and DP70 Imaging system (Olympus, Japan). Pictures of three random fields in each well were obtained from three replicate, and the number of colonies was counted.

In vitro wound healing assay
The experiment was performed as described previously with little modification [20]. Cells were cultured on Fibronectin-coated or uncoated 24-well plates in complete medium. Upon reaching confluence, the complete medium was replaced with conditioned medium (1% FBS DMEM) for an additional 24 h, and then the cell monolayer was wounded with 200 μL tips. The cells were then cultivated and photographed with a CKX41 microscope (Olympus, Japan) for another 24 h in the conditioned medium. The percent wounded area filled was calculated as follows: {(mean wounded breadth -mean remained breadth)/mean wounded breadth} × 100 (%).

Migration and invasion assays
Cell migration and invasion assays were performed using 6.5-mm trans-well chambers (8 μm pore size, Corning) as described previously with some modifications [21]. Cells were seeded at 25,000 cells per well into trans-well chambers for migration assays or at 100,000 cells per well into Matrigel-coated trans-well chambers. The wells were washed with PBS after 16 h for migration assays or after 24 h for invasion assays. The cells that had migrated to the basal side of the membrane were fixed and stained with H&E or crystal violet, visualized and photographed with a CKX41 microscope (Olympus, Japan) at 400× magnification and DP20 Imaging system (Olympus, Japan). Pictures of three random fields from three replicate wells were obtained, and the number of cells that had migrated was counted.

Cell spreading assay
The experiments were performed as previously described [21] with some modifications. Briefly, 24-well plates were pre-coated or uncoated overnight at 4°C with 10 μg/mL Human Fibronectin (Sigma) in PBS. The coated wells were washed with PBS and blocked with 1% BSA in PBS for an additional hour. Aliquots of cells (1.0 × 10 5 ) were applied to wells for various time points. After each time point, the wells were washed twice with PBS, and then the adherent cells were visualized and photographed with a CKX41 microscope (Olympus, Japan) at 200 × magnification and DP20 Imaging system (Olympus, Japan).

Immunoblotting assay
Cells were lysed and proteins were detected as described previously [10]. Immunoblotting was carried out with the anti-Fibronectin (Abcam, 1:400) and the β-actin antibody was obtained from Sigma (1:15000).

Statistics
Data are presented as the means ± SD and were evaluated with Student's t-test. P < 0.05 was accepted as statistically significant.

In vivo selection of highly metastatic human lung cancer cells
To develop an advanced metastatic model of lung cancer, we derived a highly metastatic human lung cancer cell line (named SPC-A-1sci) using the poorly metastatic human cell line SPC-A-1 as a starting point (Fig. 1A). The method used is similar to that described previously for generating a metastatic model of breast cancer [22]. Briefly, in order to get pulmonary metastatic nodules for further in vivo selection, we removed the s.c. primary tumour from tumour-bearing mice to allow sufficient time for distant micrometastases to grow into macrometastases in the first generation of mouse models. Through three rounds of in vivo serial selection, the incidence of lung metastasis reached 100% in the fourth generation of the mouse model (Table 1), from which massive metastatic lesions were isolated (Fig. 1B) and s.c. implanted into new recipient mice, and the primary tumour was adapted to tissue culture for the generation of the SPC-A-1sci cell line.
To further characterize in vivo behaviors of this cell line, we engineered SPC-A-1sci and its parent SPC-A-1 cells with dual reporters of GFP and Luciferase and monitored them in NOD/SCID mice by optical imaging techniques. As shown in Fig. 1C, we could not only detect the primary tumour growth of SPC-A-1sci cells by in vivo bioluminescence imaging weekly, but also observe the pulmonary metastases by ex vivo fluorescence imaging in one sacrificed animal at week 7 and week 8. Subsequently, numerous metastases could be detected at week 9 in all remaining mice of this group when they displayed signs of being moribund. This was significant compared with the SPC-A-1 group, in which no fluorescence signals of pulmonary metastasis could be monitored at the same end-point when they still displayed an active status (data not shown). Briefly, we successfully developed a highly metastatic cell line from a poorly metastatic lung cancer SPC-A-1 cell line. Whereas the parental line SPC-A-1 required 14 weeks (post-primary tumour resection) for the formation of visible metastatic nodules in lungs, the SPC-A-1sci required only 7 weeks for the formation of large macroscopic nodules in the lungs.

SPC-A-1sci cells display phenotypic changes consistent with EMT
The generation of motile, invasive carcinoma cells was recently found to share some of the key morphologic and molecular characteristics of EMT [23]. As shown in Fig.  2A, distinct morphological differences between the SPC-A-1sci cell line and its parental SPC-A-1 cell line were observed in cell culture. Whereas SPC-A-1 cells are polygonal and grow in cobblestone clusters, SPC-A-1sci cells are elongated and spindle-shaped, and grow in a scattering pattern to confluence. In addition, the cytoskeleton α-tubulin has also been reorganized in SPC-A-1sci cells, exhibiting the appearance of regular stretching and dispersion by immunofluorescence staining; this is not the case with SPC-A-1 cells (Fig. 2B). This morphological change is one of the hallmarks of EMT. To determine whether the molecular alterations of EMT occurred in these cells, we examined the localization of several adherent junction proteins, such as E-cadherin and ZO-1, by confocal immunofluorescence staining. The results showed that the two proteins almost disappear from the cell membrane in the SPC-A-1sci cells but show strong membrane staining in their parent SPC-A-1 cells (Fig. 2B). However, the expression levels of Ecadherin have no significant difference between SPC-A-1sci and SPC-A-1 cells by quantitative PCR analysis (Fig.  2C). Furthermore, immunohistochemical staining of s.c. primary tumours of SPC-A-1sci cells also confirmed the remarkably reduced expression of E-cadherin (Fig. 2D). In contrast, the expression of mesenchymal markers, including vimentin and fibronectin, whose expression has been shown to positively correlate with the EMT, was strongly increased in SPC-A-1sci cells (Fig. 2B-D).
EMT could be a reversible, dynamic process and may be regulated by the tumour microenvironment, carcinoma cells that have undergone EMT during invasion seem to regain their epithelial characteristics at the metastatic sites [24]. As shown in Fig. 2D, by immunohistochemical analysis, the membrane staining of the epithelial marker E-cadherin significantly increased in Figure 1 In vivo selection of highly metastatic human lung cancer cells. A, In vivo selection scheme. Parental human SPC-A-1 lung cancer cells were subcutaneously injected into NOD/SCID mice. When the subcutaneous tumour developed, small pieces of tumour tissue were implanted into the s.c. sites of new recipient mice in the first generation of mouse models, and primary tumours were excised 4 weeks later. Fourteen weeks after resection, visual lung metastases were isolated and re-implanted into the s.c. region of new recipient mice for in vivo selection. Finally, after three rounds of in vivo seleciton, tumour nodules were isolated from the lungs harboring massive metastatic lesions and s.c. implanted into new recipient mice, after which the primary tumour was removed to initiate in vitro culture and the SPC-A-1sci cell line was derived. B, Representative images of visual inspection of one mouse lungs for the presence of gross tumour nodules (arrows indicate) in the fourth generation. C, Representative bioluminescence images taken from the ventral side of the mice by in vivo BLI (upper) for primary tumour growth, and fluorescence images taken from the lungs of one sacrificed mouse by ex vivo BFI (lower) for spontaneous metastasis at three sequential time points from week 7 to 9, at 1-week intervals, after s.c. injection with GFP-Luc transduced SPC-A-1sci cells. the pulmonary metastatic nodules compared to the primary SPC-A-1sci tumour, and otherwise the mesenchymal marker, vimentin, remarkably decreased in the pulmonary metastatic nodules. Hence, both the morphological and molecular changes in SPC-A-1sci cells demonstrated that they had undergone an EMT program.

SPC-A-1sci cells acquire increased potentials of migration and invasion
Cell proliferation and colonization are among the necessary functions required for metastatic progression of tumour cells. However, the SPC-A-1sci cells are not more aggressive than the parental population in the proliferation in culture and formation of subcutaneous tumours (Fig. 3A-C). This suggests that the increased metastatic activity of SPC-A-1sci cells does not result from a faster growth rate, but rather from the acquisition of other metastasis-promoting functions.
We next took steps to characterize other cellular properties that might be relevant to metastasis. First, we employed wound healing assays to determine migratory abilities of SPC-A-1sci and SPC-A-1 cells on fibronectin coated or uncoated plates. As shown in Fig. 3D, SPC-A-1sci cells healed the wound dramatically faster than their parent cells did. It's worthy to note that this effect is not substrate dependent, as SPC-A-1sci cells also have an increased migratory ability on non-coated plate (data not shown). Importantly, when seeded on fibronectin-coated plate in this experiment, the poorly metastatic SPC-A-1

Figure 3 SPC-A-1sci cells acquire increased potentials of migration and invasion. A, In vitro growth curves of SPC-A-1sci and SPC-A-1 cells, cells
(2 × 10 4 /well) were cultured in 24-well plates, after which cells were trypsined and counted at indicated times. Results are expressed as mean ± SD; *, P < 0.05. B, Soft agar colony formation of SPC-A-1sci and SPC-A-1 cells, cells (1 × 10 3 /well) were cultured in 24-well plates, colonies were photographed and counted after three weeks. Results are expressed as mean ± SD; *, P < 0.05. C, In vivo growth curve of SPC-A-1sci and SPC-A-1 cells, cells (2.0 × 10 6 per mouse) were s.c. implanted into NOD/SCID mice, after which the tumour volume was measured over time. Results are expressed as mean ± SD. C, Motility activities of SPC-A1sci and SPC-A-1 cells were determined by wound healing assays Confluent monolayers of SPC-A1sci and SPC-A-1 cells in fibronectin coated 24-well plates were wounded by scratching, after which the wells were washed, incubated with conditioned medium for 24 h and photographed (lower). Motility was assessed on the basis of percentage of wounded area filled in (upper). Results are expressed as mean ± SD; **, P < 0.01. D and E, Trans-well migration and invasion assays of SPC-A-1sci and SPC-A-1 cells For migration assay (D), cells (2.5 × 10 4 /well) were seeded into non-coated trans-well plates and culture for 16 h at 37°C; for invasion assays (E), cells (1.0 × 10 5 /well) were seeded into Matrigel-coated trans-well plates and cultured for 24 h at 37°C, after which cells that had migrated or invaded to the underside of the inserts were stained with H&E and the cells on each insert were photographed (lower) and quantified (upper) at 400× magnification. All experiments were repeated thrice; Results are expressed as mean ± SD; *, P < 0.05. cells rapidly spread and show significantly morphologic changes. Subsequently, we also found that SPC-A-1sci cells were more randomly motile than their parent cells by trans-well migration assay (Fig. 3E). Furthermore, we determined the invasive abilities of SPC-A-1sci and SPC-A-1 cells by trans-well invasion assays, and it was found that significantly more SPC-A-1sci cells invaded to the basal side of the membrane than SPC-A-1 cells did (Fig.  3F). These data suggested that the SPC-A-1sci cells acquire enhanced abilities to migrate and invade, which may be associated with their highly metastatic behavior in vivo.

Fibronectin promotes SPC-A-1sci cell invasion and metastasis
As described above, fibronectin could promote the spreading of the parent SPC-A-1 cells. We therefore examined the spreading ability of SPC-A-1sci and SPC-A-1 cells after plating on fibronectin coated or uncoated plates and found that SPC-A-1sci cells spread significantly faster than SPC-A-1 cells did on both conditions (Fig. 4A). Importantly, SPC-A-1 cells spread rapidly and exhibited distinct morphologic changes that are similar to SPC-A-1sci cells when they were cultured on fibronectin coated plates but not on uncoated plates, such as elongated shape and increased migratory protrusions; whereas SPC-A-1sci cells showed little difference between the two conditions and maintained their mesenchymal phenotype. These findings suggested that fibronectin play a crucial role in promoting cytoskeleton reorganization and morphological transition from SPC-A-1 to SPC-A-1sci cells. In order to confirm the direct effects of fibronectin on cell migration and invasion of SPC-A-1sci cells, we used shRNA to knockdown the expression of fibronectin (Fig. 4B) and then measured the migratory and invasive abilities of SPC-A-1sci cells by trans-well assays. The results showed that knockdown of fibronectin significantly decrease the migration and invasion of SPC-A-1sci cells (Fig. 4C-D). Furthermore, the in vivo metastasis assays demonstrated the similar results. As shown in Fig. 5, not only the incidence (3/8) but also the number of lung metastatic nodules significantly decreased in mice inoculated with shRNA-Fn1 cells compared to shRNA-NC cells (8/8). These findings suggested a functional role of fibronectin in the SPC-A-1sci cell invasion and metastasis.

Discussion
Metastasis, the foremost cause of mortality in cancer patients, accounts for more than 90% of all deaths in solid tumour diseases, but the underlying mechanisms remain elusive. Recently, a combination of tumour model systems and microarray profiling technologies has been proved to be effective in identifying relevant genes and pathways for tumour metastasis [4,25]. However, most metastatic models are derived via direct injection of cancer cells into the circulation of mice, which puts the focus on end-stage metastatic cells. These models are thus likely to miss the essential genes and pathways required for the early steps of metastasis, including EMT, migration, invasion, and intravasion. It is therefore necessary to develop spontaneous metastatic models that faithfully mimic the selection and evolution of metastasis in human tumours.
Human tumours rarely metastasize from a primary tumour site in immune-deficient mice when they are transplanted into an ectopic site for the particular type of tumour being analyzed. Orthotopic implantation has been proved to be a better approach for the development of metastasis models [26]. Unlike breast cancer and melanoma, however, it is not easy to develop an orthotopic model for lung cancer because of the relative inaccessibility of anatomic location. Fortunately, s.c. tumour implantation has been widely used for the majority of human tumour models [27,28], and growing evidence shows that the incidence of metastasis can be greatly promoted by resection of primary tumours in s.c. transplantation models [22,29]. It is widely believed that metastatic cells are rare and that they evolve during the late stages of tumour progression from a series of genetic changes that enable the cells to progress through the sequential steps that finally result in growth in distant organ microenvironments [30,31]. Recently, increasing evidence has shown that highly metastatic cells have already existed in their parent cell lines or primary tumours, and the mouse could be used as a "cell sorter" to select for these cells [29,32]. In this study, we successfully isolate a highly metastatic cell line from a poorly metastatic lung cancer SPC-A-1 cell line by in vivo selection combined with resection of primary tumours in mice.
Growing evidence suggests that EMT has an important role in tumour metastasis [5,25]. The essential features of EMT are the disruption of intercellular contacts and the enhancement of cell motility, thereby leading to the release of cells from the parent epithelial tissue. The resulting mesenchymal-like phenotype is suitable for migration and thus for tumour invasion and dissemination, allowing metastatic progression to proceed. In this study, we show that SPC-A-1sci cells display morphologic changes consistent with EMT, and that some molecular alterations have taken place in these cells, such as loss of E-cadheirn from the cell membrane of SPC-A-1sci cells. Of note, there is no significant difference in the mRNA level of E-cadherin between SPC-A-1sci and SPC-A-1 cells. E-cadherin mainly binds to plasma membrane to mediate cell-cell interactions, when the membrane binding was destroyed through cleavage by γ-secretase or other proteases [33][34][35], the functions of E-cadherin would be lost even if the expression levels of it has no changes. Moreover, EMT is a reversible program, and carcinoma cells that have acquired a mesenchymal phenotype can revert to an epithelial state via a MET [36,37]. In the sequential cascade of tumour metastasis, EMT has a positive role in the majority of earlier stages; however, when the disseminating cells arrive in the microenvironment of their targeted organs, the mesenchymal phenotype is not effective in colonizing and growing into macrometastases, such that MET is required in these later stages in order to revert to an epithelial state. Our data show that, to some extent, the expression levels of some EMT markers became switched between the pulmonary metastatic nodules and the primary SPC-A-1sci tumour, as determined by immunohistochemical analysis. Those results indicate that SPC-A-1sci cells undergo EMT, which may be associated with the metastatic potential of SPC-A-1sci cells, including the enhancement of cell motility, invasion, and adhesion. However, a re-differentiation towards an epithelial phenotype, resembling a MET, is also required for the formation of pulmonary metastatic nodules in the end. These data therefore indicate that malignant progression of SPC-A-1sci cells is based on a dynamic process. A large body of literatures indicated that increased cell migration and invasion are functional hallmarks of EMT and are also important steps during the cascade of tumour metastasis [38]. Here, we showed that SPC-A-1sci cells are more aggressive than their parental cells in vitro. The potentials of cell motility, migration and invasion are greatly increased in SPC-A-1sci cells. These results suggested that SPC-A-1sci cells acquire the increased abilities to migrate and invade, which further demonstrated that SPC-A-1sci cells have undergone EMT.
The complex interactions between tumour cells and extracellular matrix play important roles in mediating and regulating many processes during tumour metastasis, including cell migration, cytoskeleton reorganization and morphologic transition [39,40]. Fibronectin is an extracellular matrix glycoprotein that plays major roles in cell differentiation, growth and migration, being involved in processes such as wound healing and embryonic development as well as oncogenic transformation [11,41]. In this study, we showed that SPC-A-1sci cells express higher level of fibronectin, and down-regulation of which can significantly decrease both the in vitro and in vivo metastatic abilities of those cells. Furthermore, fibronectin is also a mesenchymal maker, whose expression positively correlates with EMT [25]. But the contributing roles and precise mechanisms of fibronectin in EMT are still unknown. In this report, we found that fibronectin can induce the poorly metastatic SPC-A-1 cells to take on morphological changes that are similar to SPC-A-1sci cells and also typical of EMT. Briefly, these findings suggested that fibronectin play a crucial role in the highly metastatic behavior of SPC-A-1sci cells.

Conclusions
In summary, we have successfully established a reproducible s.c. mouse model and isolated a highly metastatic human lung adeno-carcinoma cell line by in vivo selection in NOD/SCID mice. The new cell line, SPC-A-1sci, possessed highly metastatic potentials and undergone an EMT program, which may be associated with increased expression of fibronectin. To the best of our knowledge, it is the first in the literature that reports the presence of EMT in lung cancer and the functional roles of fibronectin in lung cancer cell invasion and metastasis based on an in vivo tumour metastasis model. This model may provide a platform to identify elements that are important in the process of metastasis and to learn how they contribute functionally to the biology of lung cancer metastasis.