Fig. 2

Experimental validation of functional phenotypes of KDM5C in GC. A Boxplot showed the expression of KDM5C between tumor and normal samples of TCGA-STAD cohort. Statistical difference is identified by t-test, * indicating P value < 0.05; ** indicating P value < 0.01; *** indicating P value < 0.001. B Western-blotting of KDM5C in paired normal and tumor tissues of gastric cancer. C Western blotting and qPCR analyses of overexpression and known-down of KDM5C in MKN-45 cell line. Statistical difference is identified by t-test, * indicating P value < 0.05; ** indicating P value < 0.01; *** indicating P value < 0.001. D Transwell assays of MKN-45 cell line. Statistical difference is identified by t-test, * indicating P value < 0.05; ** indicating P value < 0.01; *** indicating P value < 0.001. E Cell wound scratch assays of MKN-45 cell line. Statistical difference is identified by t-test, * indicating P value < 0.05; ** indicating P value < 0.01; *** indicating P value < 0.001. F Tumor models construction using MKN-45 cell line. Statistical difference is identified by t-test, * indicating P value < 0.05; ** indicating P value < 0.01; *** indicating P value < 0.001