Fig. 2

Histologic analysis of lung metastases of WT31 and WT31_P5IV. A H&E staining of lung metastases of WT31 and WT31_P5IV. Scale bars = 200 µm B The size of lung metastases was measured and the area of macroscopic lung metastases is presented (P = 0.3512). C Representative images of immunohistochemical staining for cCasp3 of lung metastases of WT31 and WT31_P5IV are shown. Scale bars = 200 µm. D Quantification of the area of cCasp3⁺ cells in relation to the total metastatic area of lung metastases (P > 0.9999, t-test, n = 6 for WT31 and n = 5 for WT31_P5IV). E Representative images of immunohistochemical staining for Ki67 in lung metastases of WT31 and WT31_P5IV are shown. Scale bars = 200 µm. F Quantification of the area of Ki67⁺ cells in relation to the total metastatic area of lung metastases (P = 0.0171, t-test, n = 6 for WT31 and n = 5 for WT31_P5IV)