Fig. 8

The expression of ING1 and p53 proteins in CRC cell lines. The expression levels of ING1 and p53 protein as well as a loading control, β-actin, in human embryonic kidney, 293 cells, and CRC cell lines (DLD-1, Caco-2, LoVo, and HT-29) (10 μg protein of total cell extracts) were examined by western blotting using anti-human ING1 (C19, sc-7566, Santa Cruz Biotechnology) (A), anti-human p53 (DO-1, sc-126, Santa Cruz Biotechnology) (B), and anti-β-actin (C11, sc-1615, Santa Cruz Biotechnology) antibodies (C). The positions of p47-ING1 (ING1-H), p33-ING1 (ING1-L), p53, and β-actin proteins are indicated by arrows. D Quantification of the results for ING1-H, ING1-L, and p53 versus β-actin. ING1-H/β-actin and p53/β-actin were normalized to 1.0. Values are expressed as the average ± SD of 3 independent experiments. ND, not detectable