Fig. 6

Reintroduction of FANCD2 expression reverse growth suppression mediated by ATM depletion in NB cells. A Western blotting analysis of FANCD2. Ctrl and ATM-KO NGP cells (# 11 and # 13) were stably transfected with empty vector (EV) or FANCD2 expression plasmid. β-Tubulin was used as a loading control. Relative intensities of protein bands were determined using ImageJ software and normalized using loading control band intensity. B Cell proliferation assay of corresponding Ctrl and ATM-KO NGP cells. (# 11 and # 13), stably transfected with empty vector (EV) or FANCD2 expression plasmid. Error bars represent SD from three technical replicates. Statistical analysis via ordinary one-way ANOVA with Tukey’s multiple comparison test (*p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001). C Clonogenic assay of ATM-KO NGP cells (# 11 and # 13), stably transfected with EV or FANCD2 expression plasmid. Lower panel, representative images for clonogenic formation are shown. The bars represent means with SDs from three experimental replicates. Statistical significance was calculated using two-tailed paired Student’s t-test. *p < 0.05 and **p < 0.01. Corresponding uncropped full-length blots are included in Supplementary Materials