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Fig. 5 | BMC Cancer

Fig. 5

From: ATM depletion induces proteasomal degradation of FANCD2 and sensitizes neuroblastoma cells to PARP inhibitors

Fig. 5

ATM loss triggers FANCD2 degradation in a ubiquitin–proteasome dependent manner. A Total RNA was extracted from Ctrl and ATM-depleted NGP cells to detect FANCD2 mRNA by semi-quantitative RT-PCR analyses with GAPDH as an internal control. B MG132 inhibits FANCD2 degradation. Proteasome inhibition following MG132 (2 μM) treatment induced FANCD2 accumulation. Whole-cell extracts were analysed via western blotting with anti-FANCD2 and anti–β-Tubulin antibodies. Relative intensities of protein bands were determined using ImageJ software and normalized using loading control band intensity. C ATM-depleted NGP cells were treated with 2 µM MG132 for 4 h. FANCD2 was examined using fluorescence microscopy. Cell nuclei were visualized with DAPI staining. Corresponding uncropped full-length blots are included in Supplementary Materials

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