Fig. 5
Primary tumor osteopontin expression. A and B) In immunohistochemical assessments, osteopontin (red) was primarily expressed in macrophages, granulocytes, lymphocytes, and in the extracellular matrix, but also in tumor cells in the peripheral regions of tumors. C and D) Other tumors had no visible expression. E) Primary tumor osteopontin expression levels were not associated with patient sex, F) serUM-Px category, or G) nBAP-1. Similarly, in linear regressions, primary tumor osteopontin expression levels were not associated with H) primary tumor diameter (R2 = 0.001, P = 0.91), or I) serum osteopontin levels (R2 = 0.30, P = 0.08). For definition of the serUM-Px categories, see Table 2. OD, optical density. nBAP-1, nuclear BAP-1 expression level. Ns, non-significant. Scale bars: Overview 1 mm, insert 100 μm