Fig. 11

Validation of the 12 genes from the MAPS in clinical samples and cell lines. A qRT-PCR assays showed the relative mRNA expression levels of ENO2, GALNT14, HYI, PFKP, PLIN2, PLOD2, RIMKLA, and TRIB3 in sixteen paired ccRCC tumor tissues and corresponding peritumoral normal kidney tissues. B qRT-PCR assays showed the relative mRNA expression levels of ENO2, GALNT14, HYI, PFKP, PLIN2, PLOD2, RIMKLA, and TRIB3 in ccRCC cell lines (786–0, A498, and CAKI-1) and the control cell lines (HEK-293 and HK-2). C Immunohistochemistry images of ENO2, PFKP, PLIN2, TRIB3, RIMKLA, ITPKB, PLOD2, GMPPA, HYI, METAP1, GALNT7 in kidney cancer and normal kidney tissues. Examples were retrieved from the Human Protein Atlas database. P was calculated via the two-tailed Mann–Whitney test(A) and one-way ANOVA with Tukey's multiple comparisons test(B). For all the panels, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, "ns" means no significance