Table 1 Oligo sequences
Oligo name | Sequence | Description |
|---|---|---|
DNMT3A ex7 sg F | CACCGGGGGCCCGGGGAGTCTCAGA | sgRNA primer |
DNMT3A ex7 sg R | AAACTCTGAGACTCCCCGGGCCCCC | |
DNMT3A ex7 F | TTTCACGGCAAGGCAGCTGGTTG | PCR primer (445 bp) for T7e1 assay |
DNMT3A ex7 R | AGAGGAGAGCAGGACGGGAGGAG | |
DNMT3A ex23 F | GCCACCTCTTCGCTCCGCTG | RQ-PCR primer(239 bp) for clinical samples |
DNMT3A ex23 R | GATGATGTCCAACCCTTTTCGCAA | |
GUS F | GAAAATATGTGGTTGGAGAGCTCATT | RQ-PCR primer(101 bp) as internal control for clinical samples |
GUS R | CCGAGTGAAGATCCCCTTTTTA |