Fig. 8

MiR-606 targets 3’UTR of FDX1 mRNA. A qRT-PCR was used to detect the expression of miR-606 in NBT, LGG and HGG. Each value represents the mean ± SD (n = 5, each group), **P < 0.01, compared with NBT group; ^##P < 0.01, compared with LGG group. B qRT-PCR was used to detect the miR-606 expression level in NHA, U251 and U373 cells. Each value represents the mean ± SD (n = 3, each group), **P < 0.01, compared with NHA group. C qRT-PCR was used to detect the mRNA expression of FDX1 in NBT, LGG and HGG. Each value represents the mean ± SD (n = 5, each group), **P < 0.01, compared with NBT group; ^##P < 0.01, compared with LGG group. D qRT-PCR was used to detect the FDX1 mRNA expression level in NHA, U251 and U373 cells. Each value represents the mean ± SD (n = 3, each group), **P < 0.01, compared with NHA group. E Western blot was used to detect the expression of FDX1 in NBT, LGG and HGG. Each value represents the mean ± SD (n = 3, each group), **P < 0.01, compared with NBT group; ^##P < 0.01, compared with LGG group. F Western blot was used to detect the expression of FDX1 in NHA, U251 and U373. Each value represents the mean ± SD (n = 3, each group), **P < 0.01, compared with NHA group. G The predicted miR-606 binding sites in FDX1-3’UTR-Wt and the designed mutant sequence. Relative luciferase activity in HEK293T cells co-transfected with FDX1-3’UTR-Wt or FDX1-3’UTR-Mut and pre-miR-606 or pre-miR-606-NC. Data represent means ± SD (n = 3, each group). **p < 0.01 versus the FDX1-3’UTR-Wt + pre-miR-606-NC group