Fig. 5

LncRNA EGFR-AS1 facilitates the proliferation, infiltration, and migration of GC cells and promotes the expression of Ki-67, E2F1and PCNA by targeting TUBA1C. A EDU rescue assay was performed to detect proliferation of si-EGFR-AS1,si-NC and si-EGFR-AS1 + oe-TUBA1C-transfected MKN-45 cells. B Migration and invasion of si-NC, si-EGFR-AS1 or si-EGFR-AS1 + oe-TUBA1C transfected MKN-45 cells and vector,oe-EGFR-AS1,or oe-EGFR-AS1 + si-TUBA1C transfected HGC-27 cells was detected by transwell rescue assay. C The expression of KI-67, E2F1and PCNA in MKN-45 cells transfected with si-NC, si-EGFR-AS1 or si-EGFR-AS1 + oe-TUBA1C and HGC-27 cells transfected with vector,oe-EGFR-AS1,or oe-EGFR-AS1 + si-TUBA1C were detected by Western blotting.(The blots were cut prior to hybridisation with antibodies.) Data are mean ± SD. (Student’s t test **P < 0.01, ***P < 0.001 )