Fig. 4
Effects of exposure to G-CSF alone for 14 days on germ cell number in human fetal testicular tissues. Representative images of IHC staining for expression of gonocyte (AP2y, brown) and (pre)spermatogonial protein markers (MAGE-A4, blue) in control (A), 20 ng/ml G-CSF (B) or 100 ng/ml G-CSF (C) exposed human fetal testicular tissues (17GW). Dashed lines outline seminiferous cords. Quantification of total germ cell (D) and separate populations, gonocyte (E) and (pre)spermatogonial (F) numbers per cord area (mm2) in tissues exposed to G-CSF for 14 days. Each colour represents an individual fetus (n = 4; 17-22GW). Data presented as mean ± SEM and analysed using two-way ANOVA (*p < 0.05)