Table 1 Primers used for PCR analysis and according annealing temperatures
Target gene | Primer | Primer sequence (5’→3’) | Length | Accession no./ reference | Annealing temperature |
|---|---|---|---|---|---|
CDH2 (N-cadherin) | Forward Reverse | TCCACCATATGACTCCCTGTTAGT CAGAAAACTAATTCCAATCTGAAA | 454 bp | NM_001792.5 [59] | 55 °C |
GJA1 (Connexin 43) | Forward Reverse | CCATCTCTAACTCCCATGCACAGC TGGCACGACTGCTGGCTCTGCTT | 138 bp | NM_000165.5 [60] | 60 °C |
GJC1 (Connexin 45) | Forward Reverse | ATGAGTTGGAGCTTCCTGACTCG CGGCTGTTCTGTGTTGCAC | 174 bp | NM_005497.4 [61] | 65 °C |
ACTB (Beta-actin) | Forward Reverse | TTCCTTCCTGGGCATGGAGT TACAGGTCTTTGCGGATATC | 90 bp | NM_001101.5 | 60 °C |