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Fig. 7 | BMC Cancer

Fig. 7

From: Raltitrexed enhanced antitumor effect of anlotinib in human esophageal squamous carcinoma cells on proliferation, invasiveness, and apoptosis

Fig. 7

Protein expression after various treatments detected and quantified by Western blot analysis. KYSE-30 (A-H) and TE-1 (I-P) cells were treated with various treatments for 24 h. The protein levels of BAX, BCL-2, MMP-9, p-AKT, AKT, p-ERK, ERK, p-VEGFR-2, VEGFR-2, Caspase-3 and Cleaved Caspase-3 were determined by western blot analysis. β-ACTIN was detected as loading control. *P < 0.05, **P < 0.01 (versus control); #P < 0.05, ##P < 0.01 (versus 20 μM anlotinib or 2.5 μM raltitrexed)

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