Fig. 3

Combinated effects of raltitrexed and anlotinib on cell migration in ESCC cells. A and D KYSE-30 (A) and TE-1 (D) cells treated with control, 20 μM anlotinib, 2.5 μM raltitrexed or 20 μM anlotinib + 2.5 μM raltitrexed were scraped and imaged immediately (0 h) and later (24 h and 48 h), and images of the wound gap were taken. Scale bar = 100 μm. Quantitative analysis of the wound healing rate in KYSE-30 (B and C) and TE-1 (E and F) cells. Data indicate means + SD of three biological replicates. Student’s t test; ^***P < 0.001 (versus control); ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 (versus 20 μM anlotinib or 2.5 μM raltitrexed)