Fig. 2

Combinated effects of raltitrexed and anlotinib on viability of ESCC cells. A and C Colony formation ability of KYSE-30 (A) and TE-1 (C) cells inhibited by control, 20 μM anlotinib, 2.5 μM raltitrexed or 20 μM anlotinib + 2.5 μM raltitrexed. B and D Quantitative analysis of the number of colonies in KYSE-30 (B) and TE-1 (D) cells. Data indicate means + SD of three biological replicates. Student’s t test; ^***P < 0.001 (versus control); ^##P < 0.01 (versus 20 μM anlotinib or 2.5 μM raltitrexed)