Fig. 5
From: NPAS2 promotes aerobic glycolysis and tumor growth in prostate cancer through HIF-1A signaling
NPAS2 enhances glycolysis by increasing the expression of key molecules in the glycolytic pathway
(A) Western blot analysis of the expression levels of NPAS2 and key glycolysis genes in PCa cells
(B) Correlation between NPAS2 expression and key molecules of glucose metabolism in prostate cancer tissues (TCGA database)
(C) Immunohistochemical staining of NPAS2 and HIF-1A in nude mice tumors
(D) The transfection efficiency was examined with western blot (ANOVA, P<0.0001)
(E) Glucose uptake in cell transfection models (ANOVA, P = 0.007)
(F) Lactate production in cell transfection models (ANOVA, P = 0.012)
(G) Medium pH of cell transfection models (ANOVA, P = 0.036)
(H) Oxygen consumption level of cell transfection models (ANOVA, P<0.0001). *P＜0.05; **P＜0.01. Data were shown as the mean ± S.E.M. from three independent experiments