Fig. 7

The prognostic value and expression of TSC22D2. A Kaplan–Meier curve for OS between the high- and low-TSC22D2 expression group in the ICGC cohort. B CPTAC website showed the differences in TSC22D2 expression between cancer tissues and normal tissues at the protein level. C Results from GEPIA indicated the relative mRNA expression of TSC22D2 in PAAD samples and normal samples. D Bar chart shows quantification of TSC22D2 protein levels compared to GAPDH control in three independent experiments. One representative blot is shown. Error bars show standard deviation. E Assessment of relative fold change in TSC22D2 mRNA expression (HPNE set as 1) by RT-qPCR, normalized to GAPDH. Error bars show standard deviation* p < 0.05; ** p < 0.01; *** P < 0.001; **** P < 0.0001. F Hematoxylin and eosin (H&E) staining and IHC staining of TSC22D2 was performed in pancreatic cancer tissues and normal pancreatic tissues. Scale bars: low magnification, 200 μm; high magnification, 100 μm