Fig. 11

CCT3 knockdown suppressed colony formation and cell proliferation but induced apoptosis of LUAD A549 cells. (a, b) Western blotting (a) and band intensity (b) analyzes of small interfering RNA mediated CCT3 knockdown in A549 cells, GAPDH was used as the loading control. (c, d) Representative colony formation (c) and comparisons of colony counts (d) between si-CCT3 and si-Control cells. (e) The proliferation of si-CCT3 and si-Control A549 cells were determined by WST-8/Cell Counting Kit-8 at 0, 24 and 48 h after cell culturing. (f, g) Representative flow cytometry (f) and comparisons of percentages of total Annexin-V⁺ cells (g) between si-CCT3 and si-Control A549 cells. Data are representative of three independent experiments. **P < 0.01, ***P < 0.001, ns, no significance