Fig. 3

FGFR4knockdown in ccRCC cell lines. A, Immunoblot analysis of Akt/mTOR, ERK1/2, and STAT3 pathway phosphorylation after FGFR4 knockdown. B, MTS assay in ccRCC cell lines 72 h after FGFR4 knockdown. C, Morphological observation using an inverted fluorescence phase-contrast microscope before the MTS assay (left). Cells were counted using dedicated imaging software (right). Abbreviations: FGFR4, fibroblast growth factor receptor 4; RCC, renal cell carcinoma; mTOR, mammalian target of rapamycin; ERK, extracellular signal-regulated protein kinase; STAT, signal transducer and activator of transcription