Fig. 3

 H&E and immunohistochemistry (IHC) staining for substance p (SP) expression in breast cancer tissues and positive control. a). H&E staining of low SP expression in breast cancer tissue. b). H&E staining of high SP expression in breast cancer tissue. c-k). IHC staining of SP manifested mainly in the nucleus in: c). Positive control tissue (colon tissue). d). Luminal A breast cancer subtype (low expression level ≤ 5). e). Luminal A breast cancer subtype (high expression level > 5). f). HER2-enriched breast cancer subtype (low expression level ≤ 5). g). HER2-enriched breast cancer subtype (high expression level > 5). h). Triple positive breast cancer subtype (low expression level ≤ 5). i). Triple positive breast cancer subtype (high expression level > 5). j). Triple negative breast cancer subtype (low expression level ≤ 5). k). Triple negative breast cancer subtype (high expression level > 5). Arrows indicate the positively stained cells. Scale bar: 400 × (40X objective lens x 10X ocular lens = 20 μm)