Fig. 3
Purification of mouse anti-rCD44v antibodies in SDS PAGE gel (12%). Lane M, molecular weight marker. Lane 1, flow through. Lane 2, wash solution. Lanes 3, and 8, Elution buffer solution containing light and heavy chain bands of purified antibodies (about 24 and 50–70 KD) respectively (a). A spectrum of the purified antibody concentrations (8 to 0.125 µg) showed a very high absorbance value in ELISA with constant concentration (500 ng) recombinant CD44v antigen (b). Cytotoxic effects of the mouse anti-rCD44v on human breast cancer cells (MCF7 cell line) (c). Cell viability was assessed after 24 and 48 h of incubation with different concentrations (2 to 0.125 µg)by MTT assay (d). IC50 graphs of the mouse anti-rCD44v in HEK293 (e) and MCF7cells (f). Data represent the means ± SD of three independent experiments