Fig. 7

PK5-RLGal-3C induces cell apoptosis. After treated with 4 μmol/l rPK5-RL-Gal-3C for 48 h, HepG2 cells were stained with FITC-Annexin V/PI and then analyzed with flow cytometer. A and B rPK5-RL-Gal-3C treatment induced cell apoptosis on HepG2 cells. Western-blotting was performed to analyze the expression of proteins associated with cell apoptosis. C rPK5-RL-Gal-3C treatment induced protein expression of cleaved caspase-8, caspase-9, caspase-3, PARP, but down-regulated anti-apoptotic protein expression of Bcl-2. Significant differences are denoted by *** for p < 0.001