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Fig. 4 | BMC Cancer

Fig. 4

From: LncRNA ANRIL promotes HR repair through regulating PARP1 expression by sponging miR-7-5p in lung cancer

Fig. 4

PARP1 was the target gene of miR-7-5p in lung cancer. a Venn diagram shows the target genes of miR-7-5p predicted by targetscan, miRDB and starbase bioinformatics website. b Effects of ANRIL on PARP1 expression levels in lung cancer cells. c Changes in protein expression of PARP1 before and after ionizing radiation irradiation. d Change of miR-7-5p expression levels in mimic-NC-, mimic-miR-125a-3p-, inhibitor-NC- and inhibitor-miR-7-5p-transfected H1299 and H460 cells. e Changes in PARP1 protein levels after transfection of mimic-miR-7-5p or inhibitor-miR-7-5p in lung cancer cells. f Mutations were made at the putative binding site of the PARP1 3'UTR region. Luciferase activity in 293 T cells co-transfected with mimic-NC or mimic-miR-7-5p and luciferase reports containing NC, PARP1-wt or PARP1-mut transcripts. The results are presented as relative ratio of firefly luciferase activity to Renilla luciferase activity. g-h Enrichment of ANRIL and PARP1 pulled down by biotin-miR-7-5p or negative control. Error bars represent the mean ± S.D. of triplicate experiment. *p < 0.05; **p < 0.01; ***p < 0.001

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